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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has become observed in several tumors, which leads to chromosome instability, and therefore might contribute to tumorigenesis. This protein was identified to associate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a important function in marketing cell-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, are described. Two supplemental splice variants have been noted but in-depth nucleotide sequence data isn't nonetheless offered.
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Blocking peptides are peptides that bind particularly on the concentrate on antibody and block antibody binding. These peptide generally is made up of the epitope regarded from the antibody.
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This guidebook outlines more feasible optimization of mobile and immunoassay parameters to make sure the best possible effects are...
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This guidebook outlines further feasible optimization of cellular and immunoassay parameters to make certain the absolute best outcomes are...
Antibodies sure to the blocking peptide no more bind into the epitope about the focus on protein. By comparing the staining from the blocked antibody vs . the antibody on your own, you can see which staining is unique.
Target Information Cyclin E belongs to the really conserved cyclin spouse and children, whose customers are characterized by a remarkable periodicity in protein abundance through the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins show distinct expression and degradation designs which contribute to the temporal coordination of each and every mitotic function. Cyclin E types a complex with and features like a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S phase boundary which is degraded as cells progress as a result of S period.
The measurement of protein phosphorylation is a useful tool for measuring get more info the modulation of receptor activation by both of those antibodies...
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